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KMID : 0613820070170091191
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Journal of Life Science
2007 Volume.17 No. 9 p.1191 ~ p.1196
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Cooperative Induction of HL-60 Cell Differentiation by Combined Treatment with Eugenol and 1α,25-Dihydroxyvitamin D₃
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Oh Mi-Kyung
Park Seon-Joo
Kim Nam-Hoon
Cho Jin-Kyung
Jin Jong-Youl
Kim In-Sook
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Abstract
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Eugenol (4-allyl-2-methoxyphenol) is a main component of essential oils obtained from various spices. Recent reports have shown that eugenol induces growth inhibition and apoptosis of malignant tumor cells. In this study, the stimulatory effect of eugenol on cell differentiation was investigated in HL-60 promyelocytic leukemia cells. When HL-60 cells were treated in combination with 150 μM of eugenol and 3 nM of 1α,25-dihydroxyvitamin D₃, cell growth was slower than that of cells treated with eugenol or 1α,25-dihydroxyvitamin D₃ alone. Eugenol enhanced low dose of 1α,25-dihydroxyvitamin D₃-induced a G?/G₁ phase arrest in cell cycle. Consistent with this, combined treatment of eugenol and 1α,25-dihydroxyvitamin D₃ cooperatively increased p27 level and decreased cyclin A, cdk 2 and cdk 4 levels, which are cell cycle regulators related to G?/G₁ arrest. According to flow cytometric analysis, the expression of CD14 (monocytic differentiation marker) was more increased in the cells co-treated with eugenol and 1α,25-dihydroxyvitamin D₃. These results indicate that eugenol potentiates cell differentiation mediated by 1α,25-dihydroxyvitamin D₃ of suboptimal concentration. The differentiation-inducing property of eugenol maybe contributes to chemopreventive activity of cancer.
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KEYWORD
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Eugenol, 1α, 25-dihydroxyvitamin D3, cell differentiation, cell cycle, HL-60 cells
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